CASE 1: Mouse dynamic PET imaging for quantification of tracer uptake

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  • Courtesy of: D.Panetta and P.A. Salvadori, IFC-CNR Pisa, Italy

Research objective: Quantification of tracer uptake in different organs in a PET dynamic imaging

Animal model: Obese mouse (B6;129)

Acquisition protocol: 30 min acquisition time, 1 bed position

Processing and reconstruction protocol: Framing setup: 8x5” + 8x10" + 3x40" + 2x60" + 2x120" + 2x300" 3D-OSEM, 0.855 mm isotropic voxel size

Biomarker or contrast agent: Bolus injection of 18F-FDG, 6 MBq

CASE 2: Mouse PET dynamic imaging for tracer development

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  • Courtesy of: D.Panetta and P.A. Salvadori, IFC-CNR Pisa, Italy

Research objective: Dynamic PET image (MIP-coronal view) of a mouse injected with an experimental 18F-labelled tracer showing the function of renal and hepatobiliary system and subsequent uptake in the gallbladder. 

Animal model: Mouse, 20-25g

Acquisition protocol: Acquisition time: 90 min Number of bed position: 1

Processing and reconstruction protocol: 18 x 5 min frame, 250 - 750 keV 3D OSEM, 0.855 mm isotropic voxel size

Biomarker or contrast agent: Experimental 18F-labelled tracer;Injected activity: 6 MBq;Intravenously via tail vein

CASE 3: Mouse PET imaging of colorectal cancer

WT

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KO1

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KO2

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  • Courtesy of: P. Laquerriere, D.Brasse, IPHC CNRS, Strasbourg, France and A. De Arcangelis, IGBMC, Illkirch, France

Research objective:PET imaging to detect colorectal cancer and possible metastasis in a specific knocked-out mouse model. Arrows show the lesion in mice of the KO group.

Animal model:Mouse, 20-25g

Acquisition protocol:10 min acquisition time, 1 bed position

Processing and reconstruction protocol:3D-OSEM, 0.42 x 0.42 x 0.855 voxel size

Biomarker or contrast agent:Mice were injected with ~10 MBq [18F]FDG in jugular vein (i.v.). Imaging at 1 h post injection.

CASE 4: Mouse Bone PET/CT imaging using [18F]HF with Ultralow Activity

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  • Courtesy of: D.Panetta and P.A. Salvadori, IFC-CNR Pisa, Italy

Research objective:Mouse whole-body bone imaging

Animal model:Mouse, 20g

Acquisition protocol:PET: 10 min acquisition time, 1 bed position; CT: 20 s acquisition time, 80 keV, 1 mA

Processing and reconstruction protocol:PET: no frame, 3D OSEM, 0.855 mm voxel size; CT: 0.12 mm isotropic voxel size

Biomarker or contrast agent:[18F]HF, 1.7 MBq injected Imaging at 170 min post-injection (effectively only 0.58 MBq / 16 uCi in the animal during acquisition)

CASE 5: Rat PET/CT imaging with 18F-FDG

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  • Courtesy of: D.Panetta and P.A. Salvadori, IFC-CNR Pisa, Italy

Research objective:Rat PET/CT imaging with F18-FDG for accessing brain and heart uptake

Animal model:Wistar rat, 294g

Acquisition protocol:PET: 15 min acquisition time, 1 bed position; CT: 20 s acquisition time, 1 bed position, 80 kVp, 1 mA

Processing and reconstruction protocol:PET: 3D-OSEM, 0.42 x 0.42 x 0.855 mm voxel size; CT: 0.08 mm isotropic voxel size

Biomarker or contrast agent:18F-FDG, activity: 200 uCi, volume: 0.3 mL, imaging at 60 min post injection

CASE 7: Mouse PET imaging of myocardial uptake

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  • Courtesy of: P. Laquerriere, D.Brasse, IPHC CNRS, Strasbourg

Research objective:Investigating the possibility of detecting myocardial uptake of FDG without using additional cardiac gating. Results show that FDG uptakes in both left and right ventricles are clearly detectable.

Animal model:Mouse, 20-25g

Acquisition protocol:10 min acquisition time, 1 bed position

Processing and reconstruction protocol:3D-OSEM, 0.855 x 0.855 x 0.855 voxel size

Biomarker or contrast agent:Mice were injected with ~10 MBq [18F]FDG in jugular vein (i.v.). Imaging at 1 h post injection.

CASE 8: Prostate investigation using [18F]NaF

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  • Courtesy of: P. Laquerriere, D.Brasse, IPHC CNRS, Strasbourg

Research objective:The calcification (size < 1 mm) in the prostate close to the bladder could be clearly observed using [18F]NaF radiolabelled molecule. The identification of the mouse prostate is a challenging task using in vivo imaging due to its size and shape. Images were made using the IRIS PET system (Inviscan). The system demonstrates high sensitivity and excellent spatial resolution.

Animal model:Mouse, 38.7g, spontaneous model of prostate cancer developed by D. Metzger (IGBMC, Strasbourg, France)

Acquisition protocol:10 min acquisition time, 1 bed position

Processing and reconstruction protocol:3D-OSEM, 0.855 x 0.855 x 0.855 voxel size

Biomarker or contrast agent:Mice were injected with 7.6 MBq [18F]NaF.