CASE 1: Mouse dynamic PET imaging for quantification of tracer uptake

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  • Courtesy of: D.Panetta and P.A. Salvadori, IFC-CNR Pisa, Italy

Research objective: Quantification of tracer uptake in different organs in a PET dynamic imaging

Animal model: Obese mouse (B6;129)

Acquisition protocol: 30 min acquisition time, 1 bed position

Processing and reconstruction protocol: Framing setup: 8x5” + 8x10" + 3x40" + 2x60" + 2x120" + 2x300" 3D-OSEM, 0.855 mm isotropic voxel size

Biomarker or contrast agent: Bolus injection of 18F-FDG, 6 MBq

CASE 2: Mouse PET dynamic imaging for tracer development

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  • Courtesy of: D.Panetta and P.A. Salvadori, IFC-CNR Pisa, Italy

Research objective: Dynamic PET image (MIP-coronal view) of a mouse injected with an experimental 18F-labelled tracer showing the function of renal and hepatobiliary system and subsequent uptake in the gallbladder. 

Animal model: Mouse, 20-25g

Acquisition protocol: Acquisition time: 90 min Number of bed position: 1

Processing and reconstruction protocol: 18 x 5 min frame, 250 - 750 keV 3D OSEM, 0.855 mm isotropic voxel size

Biomarker or contrast agent: Experimental 18F-labelled tracer;Injected activity: 6 MBq;Intravenously via tail vein

CASE 3: Mouse PET imaging of colorectal cancer

WT

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KO1

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KO2

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  • Courtesy of: P. Laquerriere, D.Brasse, IPHC CNRS, Strasbourg, France and A. De Arcangelis, IGBMC, Illkirch, France

Research objective:PET imaging to detect colorectal cancer and possible metastasis in a specific knocked-out mouse model. Arrows show the lesion in mice of the KO group.

Animal model:Mouse, 20-25g

Acquisition protocol:10 min acquisition time, 1 bed position

Processing and reconstruction protocol:3D-OSEM, 0.42 x 0.42 x 0.855 voxel size

Biomarker or contrast agent:Mice were injected with ~10 MBq [18F]FDG in jugular vein (i.v.). Imaging at 1 h post injection.

CASE 4: Rat PET/CT imaging with 18F-FDG

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  • Courtesy of: D.Panetta and P.A. Salvadori, IFC-CNR Pisa, Italy

Research objective:Rat PET/CT imaging with F18-FDG for accessing brain and heart uptake

Animal model:Wistar rat, 294g

Acquisition protocol:PET: 15 min acquisition time, 1 bed position; CT: 20 s acquisition time, 1 bed position, 80 kVp, 1 mA

Processing and reconstruction protocol:PET: 3D-OSEM, 0.42 x 0.42 x 0.855 mm voxel size; CT: 0.08 mm isotropic voxel size

Biomarker or contrast agent:18F-FDG, activity: 200 uCi, volume: 0.3 mL, imaging at 60 min post injection

CASE 5: Mouse PET imaging of myocardial uptake

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  • Courtesy of: P. Laquerriere, D.Brasse, IPHC CNRS, Strasbourg

Research objective:Investigating the possibility of detecting myocardial uptake of FDG without using additional cardiac gating. Results show that FDG uptakes in both left and right ventricles are clearly detectable.

Animal model:Mouse, 20-25g

Acquisition protocol:10 min acquisition time, 1 bed position

Processing and reconstruction protocol:3D-OSEM, 0.855 x 0.855 x 0.855 voxel size

Biomarker or contrast agent:Mice were injected with ~10 MBq [18F]FDG in jugular vein (i.v.). Imaging at 1 h post injection.

CASE 6: Prostate investigation using [18F]NaF

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  • Courtesy of: P. Laquerriere, D.Brasse, IPHC CNRS, Strasbourg

Research objective:The calcification (size < 1 mm) in the prostate close to the bladder could be clearly observed using [18F]NaF radiolabelled molecule. The identification of the mouse prostate is a challenging task using in vivo imaging due to its size and shape. Images were made using the IRIS PET system (Inviscan). The system demonstrates high sensitivity and excellent spatial resolution.

Animal model:Mouse, 38.7g, spontaneous model of prostate cancer developed by D. Metzger (IGBMC, Strasbourg, France)

Acquisition protocol:10 min acquisition time, 1 bed position

Processing and reconstruction protocol:3D-OSEM, 0.855 x 0.855 x 0.855 voxel size

Biomarker or contrast agent:Mice were injected with 7.6 MBq [18F]NaF.

CASE 7: PET/CT cardiac imaging after myocardial ischemic injury

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  • Courtesy of: Shanxi Medical University, Taiyuan, Shanxi

Research objective: Evaluate cardiac function after myocardial ischemic injury by enhancing angiogenesis and myocardial metabolism. Investigate whether human endometrium-derived stem cells (hEMSCs) can be used for cardiac repair after ischemic injury and their possible underlying mechanisms. 

Animal model: Female nude rats (180-250g)

Acquisition protocol: Imaging start 40 min after FDG injection (~ 7 MBq). CT imaging: 20s. PET imaging: 10 min static

Image analysis: PMOD 4.0 module PCARD

CASE 8: PET/CT imaging of 68Ga-labeled nanobody companion diagnostic agents

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  • Courtesy of: H Zhao, Shanghai Renji Hospital, Shanghai

Research objective: 68Ga-labeled nanobodies were designed and investigated to track human CD8+ T cells in vivo through immuno-positron emission tomography (immunoPET).

Animal model: Nude mouse, ~ 20 g

Acquisition protocol: PET static imaging for 10 min at 0.5, 1, 1.5 and 2 h p.i. and PET dynamic images for 1 h. CT imaging for 20 s.

Biomarker or contrast agent: Injection of 3.7 MBq of 68Ga-NOTA-SNA006a, c and d.

CASE 9: Mouse PET/CT scan of 64Cu based tracer

1h post injection

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12h post injection

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48h post injection

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  • Courtesy of: C. Wang, Shanghai Renji Hospital, Shanghai

Research objective:Mouse PET/CT imaging with 64Cu labeled tracer for ovarian tumor model

Animal model:Mouse, 20.5 g

Acquisition protocol:PET/CT imaging at 1h, 12h and 48h after injection. PET imaging 10 min, CT imaging 20 s.

Biomarker or contrast agent:Injection of 5.63 MBq Cu-64-NOTA-Pertuzumab